SrasV1, Main, Exploration, bibRecord, 003131

Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions.

Identifieur interne : 003131 ( Main/Exploration ); précédent : 003130; suivant : 003132

Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions.

Auteurs : Leo L M. Poon ; J S Malik Peiris

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2008.

RBID : pubmed:19057871

Descripteurs français

KwdFr :
- Animaux, Chiroptera (virologie), Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), RT-PCR (), Reproductibilité des résultats.
MESH :
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Chiroptera.
- Animaux, Coronavirus, RT-PCR, Reproductibilité des résultats.

English descriptors

KwdEn :
- Animals, Chiroptera (virology), Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (methods).
MESH :
- classification : Coronavirus.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Chiroptera.
- Animals, Reproducibility of Results.

Abstract

The zoonotic transmission of SARS coronavirus from animals to humans revealed the potential impact of coronaviruses on mankind. This incident also triggered several surveillance programs to hunt for novel coronaviruses in human and wildlife populations. Using classical RT-PCR assays that target a highly conserved sequence among coronaviruses, we identified the first coronaviruses in bats. These assays and the cloning and sequencing of the PCR products are described in this chapter. Using the same approach in our subsequent studies, we further detected several novel coronaviruses in bats. These findings highlighted the fact that bats are important reservoirs for coronaviruses.

DOI: 10.1007/978-1-59745-181-9_2
PubMed: 19057871

Affiliations:

Le document en format XML

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<term>Coronavirus (isolation & purification)</term>
<term>Reproducibility of Results</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
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<term>Coronavirus ()</term>
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Serveur d'exploration SRAS

Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions.

Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
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